Wash your hands well before brushing your teeth and handling your brush. Most people have the habit of reaching for their brush before washing hands which transmits germs and bacteria. Wash your hands well and rinse the brush with a pressure from your thumb on the grains. Hold it under the running water to get rid of any hidden particles. Use your thumb to move back and forth over the bristles to expose any hidden contamination.
Fill the quarter of a cup with vinegar. Place your brush in it with the bristles dipped in the vinegar. Leave it overnight. The vinegar will act as a cleansing agent and kill all the live contaminants on your brush. Do rinse your brush well after this process to clear away the taste of the vinegar.
Prepare a solution of water and your mouth wash, preferably an antibacterial one. Dip your toothbrush into it and swish it around for a good exposure. Do this for a minute and your brush will be free of any bacteria.
Salt solution does a great job on your brush. Prepare a salt and water solution in a glass. Now place the brush inside it upside down. Leave it like this for a couple of hours. Let the salt play its magic over the brush. Take the brush out and rinse well with warm water.